离子在人类淋巴细胞激活中的作用。

Role of ions in activation of human lymphocytes.

作者信息

Pearson J L, Ray M

出版信息

Can J Genet Cytol. 1983 Dec;25(6):670-5. doi: 10.1139/g83-099.

Abstract

Phytohemagglutinin (PHA)-stimulated lymphocytes were cultured in media containing varying levels of K+, Mg2+, Ca2+. Cell activation was monitored by measuring nuclear diameter and by evaluating the area of nucleolus which reacted with silver nitrate. Decreasing extracellular K+ from normal levels (5.0 mM) to 14% (0.7 mM) and decreasing extracellular Mg2+ from normal levels (1.0 mM) to 14% (0.14 mM) did not affect nuclear diameter or silver nitrate reactivity of PHA-stimulated lymphocytes. Chelation of extracellular Ca2+ with EGTA during the first 24 h after PHA stimulation completely inhibited the increases in silver reactivity and nuclear diameter associated with stimulation. Chelation of extracellular Ca2+ 48 h after PHA stimulation did not inhibit lymphocyte stimulation. Inhibitory effects of EGTA were completely reversed if CaCl2 was added to the medium within 24 h of PHA stimulation. By 48 h the effects were irreversible.

摘要

将植物血凝素（PHA）刺激的淋巴细胞培养于含有不同水平钾离子（K⁺）、镁离子（Mg²⁺）、钙离子（Ca²⁺）的培养基中。通过测量细胞核直径以及评估与硝酸银反应的核仁面积来监测细胞活化情况。将细胞外钾离子从正常水平（5.0 mM）降至14%（0.7 mM），以及将细胞外镁离子从正常水平（1.0 mM）降至14%（0.14 mM），均未影响PHA刺激的淋巴细胞的细胞核直径或硝酸银反应性。在PHA刺激后的最初24小时内，用乙二醇双四乙酸（EGTA）螯合细胞外钙离子，完全抑制了与刺激相关的银反应性和细胞核直径的增加。PHA刺激48小时后螯合细胞外钙离子并未抑制淋巴细胞刺激。如果在PHA刺激后24小时内将氯化钙添加到培养基中，EGTA的抑制作用会完全逆转。到48小时时，这些作用是不可逆的。