Cohen P L, Eisenberg R A, Dermer G B
Clin Exp Immunol. 1984 Mar;55(3):509-15.
The sera of autoimmune and other mouse strains were analysed by high resolution two dimensional gel electrophoresis and silver staining. The most striking finding was the presence of unusually acidic immunoglobulin light chains in sera from 4-5 month old MRL/Mp-lpr/lpr mice. Similar light chains were found in lesser amounts in NZB/NZW and BXSB sera, and traces were observed in MRL/Mp-+/+, NZB, and in normal sera. The intensity of the acidic light chain spots increased with age in MRL/Mp-lpr/lpr mice, coincident with the development of autoimmunity. These acidic light chains circulated as components of complete IgG molecules, as they were no longer demonstrable in serum which had been rendered free of IgG by absorption with Cowans strain staphylococci. Because both cryoglobulins and glomerular eluates from MRL/Mp-lpr/lpr mice contained acidic immunoglobulin light chains, these molecules may be involved in immune complex formation and resultant injury.
通过高分辨率二维凝胶电泳和银染分析自身免疫及其他小鼠品系的血清。最显著的发现是，4至5月龄的MRL/Mp-lpr/lpr小鼠血清中存在异常酸性的免疫球蛋白轻链。在NZB/NZW和BXSB血清中也发现了少量类似的轻链，在MRL/Mp-+/+、NZB血清以及正常血清中观察到了痕量。在MRL/Mp-lpr/lpr小鼠中，酸性轻链斑点的强度随年龄增加，这与自身免疫的发展相一致。这些酸性轻链作为完整IgG分子的成分循环，因为在用考恩斯菌株葡萄球菌吸收使血清中不含IgG后，它们就不再能被检测到。由于MRL/Mp-lpr/lpr小鼠的冷球蛋白和肾小球洗脱液中都含有酸性免疫球蛋白轻链，这些分子可能参与免疫复合物的形成及由此导致的损伤。
