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关于肿瘤细胞体外表面结构的柔韧性

On the flexibility of surface structures of tumor cells in vitro.

作者信息

Paweletz N, Finze E M

出版信息

In Vitro. 1984 Feb;20(2):103-8. doi: 10.1007/BF02626650.

DOI:10.1007/BF02626650
PMID:6423516
Abstract

When HeLa cells are detached from their support by trypsin, trypsin-EDTA, or by mechanical means large zeiotic blebs are formed. After reseeding the cells onto glass these blebs shrink. Those blebs near the support collapse completely to form lobes in the neighborhood of lobopodia. By scanning and transmission electron microscopy we could show the transformation of these zeiotic blebs into true lobopodia. This implies a repair of cell surface structures and components as well as of the subcortical cytoskeleton.

摘要

当用胰蛋白酶、胰蛋白酶 - 乙二胺四乙酸(EDTA)或通过机械手段使海拉细胞与其支撑物分离时,会形成大的游动泡。将细胞重新接种到玻璃上后,这些泡会收缩。靠近支撑物的那些泡会完全塌陷,在伪足附近形成叶状结构。通过扫描电子显微镜和透射电子显微镜,我们能够展示这些游动泡向真正伪足的转变。这意味着细胞表面结构和成分以及皮层下细胞骨架的修复。

相似文献

1
On the flexibility of surface structures of tumor cells in vitro.关于肿瘤细胞体外表面结构的柔韧性
In Vitro. 1984 Feb;20(2):103-8. doi: 10.1007/BF02626650.
2
Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture.
Exp Cell Res. 1978 May;113(2):345-57. doi: 10.1016/0014-4827(78)90375-0.
3
Conversion of blebs to microvilli: cell surface reorganisation after trypsin.疱状突起向微绒毛的转变:胰蛋白酶作用后的细胞表面重组
Differentiation. 1981;20(2):168-73. doi: 10.1111/j.1432-0436.1981.tb01172.x.
4
Alteration of the cell membrane architecture during suspension and monolayer culturing.悬浮培养和单层培养过程中细胞膜结构的改变。
Int J Cancer. 1976 Mar 15;17(3):407-15. doi: 10.1002/ijc.2910170318.
5
Cell coat in tumor cells -- effects of trypsin and EDTA: a biochemical and morphological study.肿瘤细胞的细胞被膜——胰蛋白酶和乙二胺四乙酸的作用:一项生物化学与形态学研究。
Oncology. 1976;33(1):17-23. doi: 10.1159/000225095.
6
Reversible changes in nuclear and cell surface topography in cells exposed to collagenase and EDTA.暴露于胶原酶和乙二胺四乙酸(EDTA)的细胞中细胞核和细胞表面拓扑结构的可逆变化。
Cell Tissue Res. 1981;220(1):51-60. doi: 10.1007/BF00209965.
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Influence of proteolytic enzymes and calcium-binding agents on nuclear and cell surface topography.蛋白水解酶和钙结合剂对细胞核及细胞表面形貌的影响。
Cell Tissue Res. 1984;237(3):587-93. doi: 10.1007/BF00228443.
8
Cytoskeleton as a target in menadione-induced oxidative stress in cultured mammalian cells: alterations underlying surface bleb formation.细胞骨架作为甲萘醌诱导的培养哺乳动物细胞氧化应激的靶点:表面气泡形成的潜在改变
Chem Biol Interact. 1991;80(2):217-36. doi: 10.1016/0009-2797(91)90026-4.
9
On the dynamics of the microfilament system in HeLa cells.关于海拉细胞中微丝系统的动力学
J Cell Biol. 1982 Apr;93(1):122-8. doi: 10.1083/jcb.93.1.122.
10
Assessment of attachment of Neisseria gonorrhoeae to HeLa cells by double radiolabeling.通过双重放射性标记评估淋病奈瑟菌对HeLa细胞的黏附。
Infect Immun. 1979 Sep;25(3):1043-50. doi: 10.1128/iai.25.3.1043-1050.1979.

本文引用的文献

1
Conversion of blebs to microvilli: cell surface reorganisation after trypsin.疱状突起向微绒毛的转变:胰蛋白酶作用后的细胞表面重组
Differentiation. 1981;20(2):168-73. doi: 10.1111/j.1432-0436.1981.tb01172.x.
2
Surface morphology of trypsinized human cells in vitro.体外胰蛋白酶消化的人细胞的表面形态。
Exp Cell Res. 1971 Jun;66(2):353-61. doi: 10.1016/0014-4827(71)90688-4.
3
Changes in surface morphology of Chinese hamster ovary cells during the cell cycle.中国仓鼠卵巢细胞在细胞周期中的表面形态变化。
J Cell Biol. 1973 Jun;57(3):815-36. doi: 10.1083/jcb.57.3.815.
4
Trypsin-induced coordinate alterations in cell shape, cytoskeleton, and intrinsic membrane structure of contact-inhibited cells.
Exp Cell Res. 1978 Jun;114(1):1-14. doi: 10.1016/0014-4827(78)90029-0.