Paweletz N, Finze E M
In Vitro. 1984 Feb;20(2):103-8. doi: 10.1007/BF02626650.
When HeLa cells are detached from their support by trypsin, trypsin-EDTA, or by mechanical means large zeiotic blebs are formed. After reseeding the cells onto glass these blebs shrink. Those blebs near the support collapse completely to form lobes in the neighborhood of lobopodia. By scanning and transmission electron microscopy we could show the transformation of these zeiotic blebs into true lobopodia. This implies a repair of cell surface structures and components as well as of the subcortical cytoskeleton.
当用胰蛋白酶、胰蛋白酶 - 乙二胺四乙酸(EDTA)或通过机械手段使海拉细胞与其支撑物分离时,会形成大的游动泡。将细胞重新接种到玻璃上后,这些泡会收缩。靠近支撑物的那些泡会完全塌陷,在伪足附近形成叶状结构。通过扫描电子显微镜和透射电子显微镜,我们能够展示这些游动泡向真正伪足的转变。这意味着细胞表面结构和成分以及皮层下细胞骨架的修复。