Anglhileri L J
Oncology. 1976;33(1):17-23. doi: 10.1159/000225095.
The effects of trypsin and EDTA on the cell coat of ascites tumor cells were studied by means of biochemical and electron microscopy techniques. EDTA seems to release, by chelation of the Ca2+-bridges, an outer layer of glycoproteins of presumably exogenous origin. On the contrary, trypsin produces a deeper enzymic cleavage which appears to affect the structural integrity of the bilayered cell membrane. The significance of the cell leakage in tumor cells and the effect of EDTA on the modification of this leakage by change of cell membrane permeability are discussed.
采用生化和电子显微镜技术研究了胰蛋白酶和乙二胺四乙酸（EDTA）对腹水肿瘤细胞细胞被膜的影响。EDTA似乎通过螯合Ca2+桥释放出一层可能来自外源的糖蛋白外层。相反，胰蛋白酶会产生更深层次的酶解作用，这似乎会影响双层细胞膜的结构完整性。文中讨论了肿瘤细胞中细胞渗漏的意义以及EDTA通过改变细胞膜通透性对这种渗漏修饰的影响。
