分离的破骨细胞中的碳酸酐酶活性。

Carbonic anhydrase activity in isolated osteoclasts.

作者信息

Gay C V, Ito M B, Schraer H

出版信息

Metab Bone Dis Relat Res. 1983;5(1):33-9. doi: 10.1016/0221-8747(83)90048-6.

Abstract

Osteoclasts were isolated from the long bones of chicks by a nylon mesh filtering system. The cell purity, in terms of area of the slide occupied, was on the average 77.5% osteoclasts, 22% aggregated osteoblasts and matrix debris, and 1.5% individual blood and marrow cells. Viability, as measured by cytochalasin-blockable phagocytosis, of up to 99% was obtained. Electron microscopic examination revealed good retention of ultrastructural features. The presence of carbonic anhydrase and acid phosphatase in osteoclasts was verified by selective staining methods; the aggregates were positive for alkaline phosphatase. Carbonic anhydrase activity was 0.89 +/- 0.13 X 10(-4) micro Wilbur-Anderson units per osteoclast, and red blood cells had 0.12 +/- 0.03 X 10(-4) units/cell. Neither calcitonin nor parathyroid hormone influenced the activity of carbonic anhydrase. A review of other hormonal effects on carbonic anhydrase is provided.

摘要

通过尼龙网过滤系统从小鸡的长骨中分离出破骨细胞。就所占载玻片面积而言，细胞纯度平均为77.5%的破骨细胞、22%的聚集成骨细胞和基质碎片以及1.5%的单个血细胞和骨髓细胞。通过细胞松弛素可阻断的吞噬作用测定，细胞活力高达99%。电子显微镜检查显示超微结构特征保留良好。通过选择性染色方法证实破骨细胞中存在碳酸酐酶和酸性磷酸酶；聚集物碱性磷酸酶呈阳性。每个破骨细胞的碳酸酐酶活性为0.89±0.13×10⁻⁴微威尔伯 - 安德森单位，红细胞的活性为0.12±0.03×10⁻⁴单位/细胞。降钙素和甲状旁腺激素均不影响碳酸酐酶的活性。本文还综述了其他激素对碳酸酐酶的影响。