Ali N N, Boyde A, Jones S J
Anat Embryol (Berl). 1984;170(1):51-6. doi: 10.1007/BF00319457.
Rabbit osteoclasts (OCs), separated mechanically from long bones, were seeded on to glass or plastic substrates or slabs of sperm whale dentine (SWD). Cells were cultured in MEM with 10% FCS with or without added salmon calcitonin (SCT) at dosages of 0.001, 0.1 and 1 IU/ml. Although most rabbit SCT-treated OCs on the non-biological substrates showed inhibition of peripheral ruffling activity and motility at dosages that stop rat OC movement, resorption still occurred on the dentine. Thus such inhibition is unreliable as a general indicator for resorptive capability. Resorption lacunae were observed at all times from 6 h onwards. Using stereophotogrammetric techniques, the following minimum values were obtained from 24 h cultures: highest hourly rate of resorption of dentine for single OC, 570 micron3/h; average rate 165 micron3/h; mean total volume dentine removed per Howship's lacuna complex, 3,885 micron3; average value for plan area of surface attacked per OC, 1,450 micron2.
从兔长骨中机械分离出破骨细胞（OCs），接种到玻璃、塑料基质或抹香鲸牙质（SWD）薄片上。细胞在含有10%胎牛血清（FCS）的MEM培养基中培养，添加或不添加剂量为0.001、0.1和1 IU/ml的鲑鱼降钙素（SCT）。尽管在非生物基质上，大多数经兔SCT处理的破骨细胞在能抑制大鼠破骨细胞运动的剂量下，其周边褶皱活动和运动受到抑制，但在牙质上仍会发生吸收。因此，这种抑制作为吸收能力的一般指标并不可靠。从6小时起随时都能观察到吸收陷窝。使用立体摄影测量技术，从24小时培养物中获得了以下最小值：单个破骨细胞对牙质的最高每小时吸收率为570立方微米/小时；平均速率为165立方微米/小时；每个霍氏陷窝复合体去除的牙质平均总体积为3885立方微米；每个破骨细胞攻击的表面平面面积平均值为1450平方微米。
