生长激素释放因子调节大鼠垂体细胞原代培养物中的生长激素信使核糖核酸。
Growth hormone-releasing factor regulates growth hormone mRNA in primary cultures of rat pituitary cells.
作者信息
Gick G G, Zeytin F N, Brazeau P, Ling N C, Esch F S, Bancroft C
出版信息
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1553-5. doi: 10.1073/pnas.81.5.1553.
Abstract
A peptide with high intrinsic activity for specifically stimulating the secretion of immunoreactive growth hormone (GH; somatotropin) has been characterized and reproduced by total synthesis. This peptide, human pancreatic growth hormone-releasing factor, 44-amino-acid form (hpGRF1-44-NH2), was isolated from a tumor localized in the pancreas of a patient with acromegaly. We report here the effect of this growth hormone-releasing factor (GRF) on GH release and the GH mRNA levels in monolayer cultures of rat pituitary. The cytoplasmic dot hybridization technique was used to examine the effect of GRF on GH mRNA levels. Incubation of rat pituitary cultures with GRF for 72 hr resulted in a 2- to 2.5-fold increase in GH mRNA levels, and the maximal levels of stimulation were achieved at GRF concentrations as low as 1 fM. GRF did not stimulate prolactin release, nor did it affect specific prolactin mRNA levels in the pituitary cultures. We conclude that GRF is a potent and specific GH secretagogue that also affects specifically GH mRNA levels in normal pituitary cells.
摘要
一种具有高内在活性、能特异性刺激免疫反应性生长激素（GH；促生长激素）分泌的肽已被鉴定，并通过全合成得以重现。这种肽，即人胰腺生长激素释放因子44氨基酸形式（hpGRF1 - 44 - NH2），是从一名肢端肥大症患者胰腺中的肿瘤分离出来的。我们在此报告这种生长激素释放因子（GRF）对大鼠垂体单层培养物中GH释放及GH mRNA水平的影响。采用细胞质斑点杂交技术检测GRF对GH mRNA水平的影响。用GRF孵育大鼠垂体培养物72小时，导致GH mRNA水平增加2至2.5倍，且在低至1 fM的GRF浓度下即可达到最大刺激水平。GRF不刺激催乳素释放，也不影响垂体培养物中特异性催乳素mRNA水平。我们得出结论，GRF是一种强效且特异性的GH促分泌素，它也能特异性影响正常垂体细胞中的GH mRNA水平。
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本文引用的文献
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Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.细胞质斑点杂交。对多个小细胞或组织样本中相对mRNA水平的简单分析。
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Thyroid or glucocorticoid hormone induces pre-growth-hormone mRNA and its probable nuclear precursor in rat pituitary cells.甲状腺激素或糖皮质激素可诱导大鼠垂体细胞中的前生长激素信使核糖核酸及其可能的核前体。
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Somatocrinin, growth hormone releasing factor, stimulates secretion of growth hormone in anesthetized rats.生长激素释放素，即生长激素释放因子，可刺激麻醉大鼠分泌生长激素。
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