完整人血小板的脂氧合酶活性

Lipoxygenase activity of intact human platelets.

作者信息

Lagarde M, Croset M, Boukhchache D, Greffe A, Dechavanne M, Renaud S

出版信息

Prostaglandins Leukot Med. 1984 Jan;13(1):61-6. doi: 10.1016/0262-1746(84)90103-3.

Abstract

The oxygenation by lipoxygenase of different icosaenoic and docosaenoic acids by intact human platelets was studied. The HPLC analysis of the hydroxy compound (s) derived from icosaenoic acids showed that the 12-derivatives predominate. The increase of the fatty acid concentration markedly enhanced their oxygenation except for icosapentaenoic acid. The conversion of this acid into its hydroxy derivative rose in the presence of arachidonic acid, probably through both its cyclo-oxygenase and lipoxygenase product formation. Since 12-hydroxy-icosaenoic acids are modulators of PGH2-induced platelet aggregation, we conclude that the interactions between polyunsaturated fatty acids during their oxygenation by platelet lipoxygenase could be relevant to the regulating activity of dietary fatty acids.

摘要

研究了完整人血小板中脂氧合酶对不同二十碳烯酸和二十二碳烯酸的氧化作用。对源自二十碳烯酸的羟基化合物进行的高效液相色谱分析表明，12-衍生物占主导。除二十碳五烯酸外，脂肪酸浓度的增加显著增强了它们的氧化作用。在花生四烯酸存在的情况下，该酸向其羟基衍生物的转化增加，可能是通过其环氧化酶和脂氧合酶产物的形成。由于12-羟基二十碳烯酸是PGH2诱导的血小板聚集的调节剂，我们得出结论，血小板脂氧合酶氧化过程中多不饱和脂肪酸之间的相互作用可能与膳食脂肪酸的调节活性有关。