Hofer E
Zentralbl Bakteriol Mikrobiol Hyg A. 1983 Dec;256(2):175-83.
The incidence of Listeria monocytogenes in 61 fresh stool specimens from normal beef cattle obtained from a slaughterhouse in Rio de Janeiro was studied. The animals were divided in two groups of similar number separated by age; animals of up to five years, and animals of 5 years and more. In the research of this microorganism 9 culture media were used: four enrichment media, one for transport and four selective media. From the results, 11 animals proved to be carriers, of which nine were five years of age or older and two were of the younger group. Two hundred and twenty-one samples of Listeria were obtained from these 11 animals. The combination of Tryptose broth containing 40 mcg/ml nalidixic acid and 10 mcg/ml trypaflavine, incubated for 5 days at ambient temperature (+/- 28 degrees C), with the second modification of Ralovich medium, showed the best results for detection of Listeria both quantitatively and qualitatively. Among the serotypes characterized in this research the types with greater incidence were L 4 a (60.18%), L 4 f (20.36%), and L 4 b (9.05%).
对从里约热内卢一家屠宰场获取的61份正常肉牛新鲜粪便样本中的单核细胞增生李斯特菌发病率进行了研究。这些动物按年龄分为两组，数量相近；五岁及以下的动物为一组，五岁及以上的动物为另一组。在对这种微生物的研究中，使用了9种培养基：4种增菌培养基、1种运送培养基和4种选择性培养基。结果显示，11只动物被证实为带菌者，其中9只为五岁及以上，2只为较年轻组。从这11只动物身上获取了221份李斯特菌样本。含有40 mcg/ml萘啶酸和10 mcg/ml曲帕沙星的胰蛋白胨肉汤，在环境温度（±28摄氏度）下培养5天，并与拉洛维奇培养基的第二种改良培养基结合使用，在定量和定性检测李斯特菌方面均显示出最佳效果。在本研究中鉴定的血清型中，发病率较高的类型为L 4 a（60.18%）、L 4 f（20.36%）和L 4 b（9.05%）。
