枯草芽孢杆菌胞外蔗糖转化酶前体形式的表征
Characterization of the precursor form of the exocellular levansucrase from Bacillus subtilis.
作者信息
Fouet A, Arnaud M, Klier A, Rapoport G
出版信息
Biochem Biophys Res Commun. 1984 Mar 15;119(2):795-800. doi: 10.1016/s0006-291x(84)80320-4.
PMID:6424671
Abstract
Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.
摘要
通过对粗提物中的酶进行测定、SDS-聚丙烯酰胺凝胶电泳和免疫印迹分析，在大肠杆菌微细胞中证实了克隆的果聚糖蔗糖酶基因（sacB）的表达。还通过与该蛋白质NH2末端区域相对应的DNA序列证实并确认了分子量为53000的酶前体形式的存在。
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