解淀粉芽孢杆菌果聚糖蔗糖酶编码基因的分离与鉴定
Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens.
作者信息
Tang L B, Lenstra R, Borchert T V, Nagarajan V
机构信息
Central Research and Development Division, E.I. duPont de Nemours Company, Wilmington, DE 19880-0228.
出版信息
Gene. 1990 Nov 30;96(1):89-93. doi: 10.1016/0378-1119(90)90345-r.
PMID:2265762
Abstract
The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis. Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region. The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
摘要
从解淀粉芽孢杆菌中分离出编码果聚糖蔗糖酶（LVS）的基因（sacB[BamP]），进行测序并在枯草芽孢杆菌中表达。对sacB[BamP]核苷酸序列的分析表明，其在启动子和编码区与枯草芽孢杆菌LVS编码基因具有广泛的同源性。克隆到多拷贝质粒中的sacB[BamP]基因在枯草芽孢杆菌中受蔗糖诱导。
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