Ronai A, Berning W, Gaa A, von Deimling O
Abteilung für Chemische Pathologie, Universität Freiburg, Bundesrepublik Deutschland.
Biochem Genet. 1993 Aug;31(7-8):279-94.
A new carboxylesterase isozyme (EC 3.1.1.1), designated ES-30, is described in mouse liver. Two phenotypes were distinguished, ES-30A, a possible null type, was found in SPE/Pas and in other lines derived from Mus spretus, and ES-30B was found in BALB/cJ and other laboratory inbred strains. ES-30B is characterized by a distinct electrophoretic band when stained using 5-bromoindoxyl acetate as the substrate. After isolation and purification from other esterases by ion-exchange chromatography and molecular sieving, the molecular mass was estimated by two independent methods to be 62 and 64 kDa, respectively. The activity of ES-30B is higher in adult males than in females and can be stimulated in vivo by testosterone. The distribution of phenotypes on the progeny of a backcross series suggests a separate locus, Es-30, with the allele a for absence and b for presence of the isozyme. Locus Es-30 is shown to be closely linked to Es-2 and to Es-7 of cluster-2 on chromosome 8. The gene order Es-9--Got-2--(Es-2, Es-7, Es-30) is suggested.
在小鼠肝脏中发现了一种新的羧酸酯酶同工酶（EC 3.1.1.1），命名为ES-30。区分出了两种表型，ES-30A可能是无效型，在SPE/Pas以及源自斯氏小家鼠的其他品系中发现，ES-30B在BALB/cJ和其他实验室近交系中发现。当以乙酸5-溴吲哚酚作为底物染色时，ES-30B的特征是有一条明显的电泳带。通过离子交换色谱法和分子筛从其他酯酶中分离纯化后，用两种独立方法估计其分子量分别为62 kDa和64 kDa。ES-30B在成年雄性中的活性高于雌性，并且在体内可被睾酮刺激。回交系列后代的表型分布表明存在一个单独的基因座Es-30，等位基因a表示该同工酶缺失，b表示存在。已证明基因座Es-30与8号染色体上簇2的Es-2和Es-7紧密连锁。推测基因顺序为Es-9--Got-2--(Es-2, Es-7, Es-30)。
