Itoh M, Ishihara J, Itagaki A, Taniguchi K, Miyai K, Kurimura T
Biken J. 1983 Sep;26(3):121-5.
An enzyme-linked immunosorbent assay (ELISA) system for hepatitis B e antigen (HBeAg) was developed employing beta-D-galactosidase conjugated with antibody to HBeAg (anti-HBe) and using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the coupling reagent. The experimental conditions for quantitative assay of HBeAg were determined. The presence of rheumatoid factor in test sera did not affect the results. This assay system is more sensitive than the micro-Ouchterlony method and as sensitive as radioimmunoassay. The use of beta-D-galactosidase for ELISA in the field of virology is recommended.
利用与乙型肝炎e抗原抗体（抗-HBe）偶联的β-D-半乳糖苷酶，以间马来酰亚胺苯甲酰-N-羟基琥珀酰亚胺酯作为偶联试剂，开发了一种用于检测乙型肝炎e抗原（HBeAg）的酶联免疫吸附测定（ELISA）系统。确定了定量检测HBeAg的实验条件。检测血清中类风湿因子的存在不影响结果。该检测系统比微量双向免疫扩散法更灵敏，与放射免疫测定法灵敏度相当。建议在病毒学领域将β-D-半乳糖苷酶用于ELISA检测。
