Akiyama K
Jpn J Exp Med. 1983 Aug;53(4):181-5.
A highly sensitive enzyme-linked immunosorbent assay (ELISA) procedure has been devised for the determination of anti-myelin basic protein antibodies. First, rabbit anti-myelin basic protein (MBP) sera to be assayed were incubated with MBP immobilized on small pieces of silicone rubber. Next, anti-MBP bound specifically to the solid phase was allowed to react either with Fab' of guinea pig anti-rabbit IgG conjugated with beta-D-galactosidase from Escherichia coli or with protein A conjugated with beta-D-galactosidase. The amount of fixed anti-MBP antibodies was assayed by fluorometric determination of the enzyme activity using 4-methylumbelliferyl-beta-D-galactoside as a substrate. The sensitivity of the present assay was at least an order of magnitude higher than the hitherto available radioimmunoassay and ELISA.
已设计出一种高灵敏度的酶联免疫吸附测定（ELISA）方法来测定抗髓鞘碱性蛋白抗体。首先，将待检测的兔抗髓鞘碱性蛋白（MBP）血清与固定在小块硅橡胶上的MBP一起孵育。接下来，使特异性结合到固相上的抗MBP与与来自大肠杆菌的β-D-半乳糖苷酶偶联的豚鼠抗兔IgG的Fab'或与与β-D-半乳糖苷酶偶联的蛋白A反应。使用4-甲基伞形酮基-β-D-半乳糖苷作为底物，通过荧光测定酶活性来检测固定的抗MBP抗体的量。本测定方法的灵敏度比迄今可用的放射免疫测定和ELISA至少高一个数量级。
