Richter F, Böhme H J, Hofmann E
Biomed Biochim Acta. 1983;42(10):1229-35.
The isozyme distribution of glycogen phosphorylase b was studied in various fetal, neonatal, and adult rat tissues by means of discontinuous polyacrylamide gel electrophoresis in the presence of glycogen in the separating gel. The brain-type isozyme BB being the predominant form in fetal rat tissues is replaced during maturation either partially (brain and kidney) or completely (skeletal muscle and liver) by the isozymes MM or LL, respectively. In the various organs, the developmental formation of the adult phosphorylase isozyme pattern does not proceed simultaneously. The definitive isozyme pattern is expressed in heart muscle at the 19th day of gestation. The isozyme transitions in liver and skeletal muscle are finished about two days after birth, while at this time in the brain just the muscle-type subunit M appears and forms the hybrid MB. The isozyme distribution of the maturated rat brain (BB, MB, MM) is not expressed before the 9th day of postnatal life.
在分离胶中存在糖原的情况下，通过不连续聚丙烯酰胺凝胶电泳研究了糖原磷酸化酶b在不同胎龄、新生和成年大鼠组织中的同工酶分布。脑型同工酶BB是胎鼠组织中的主要形式，在成熟过程中，分别被MM或LL同工酶部分（脑和肾）或完全（骨骼肌和肝）取代。在各个器官中，成年磷酸化酶同工酶模式的发育形成并非同时进行。在妊娠第19天时，心肌中出现了确定的同工酶模式。肝和骨骼肌中的同工酶转变在出生后约两天完成，而此时脑中仅出现肌肉型亚基M并形成杂合MB。成熟大鼠脑（BB、MB、MM）的同工酶分布在出生后第9天才表现出来。
