Richter F, Böhme H J, Hofmann E
Institute of Biochemistry, Karl-Marx University, School of Medicine, Leipzig, GDR.
Biomed Biochim Acta. 1988;47(8):743-52.
Quantitative estimates of M- and L-type phosphorylase subunits in adult and developing rat tissues have been obtained by immunotitration of tissue extracts with subunit-specific antibodies. It has been shown that M-type subunits occur in all studied tissues with exception of adult liver and placenta. L-type subunits account for most of the total phosphorylase activity in liver and bone marrow but are present also in a variety of other adult and in most of the fetal tissues examined. During maturation, selective increases in the expression of phosphorylase isozymes occur in certain tissues such as liver, brain, heart and skeletal muscle. The hitherto unknown hybrid LM has been produced by in vitro hybridization of M- and L-subunits.
通过用亚基特异性抗体对组织提取物进行免疫滴定，已获得成年和发育中大鼠组织中M型和L型磷酸化酶亚基的定量估计值。结果表明，除成年肝脏和胎盘外，M型亚基存在于所有研究的组织中。L型亚基占肝脏和骨髓中总磷酸化酶活性的大部分，但也存在于多种其他成年组织以及大多数所检查的胎儿组织中。在成熟过程中，某些组织如肝脏、大脑、心脏和骨骼肌中磷酸化酶同工酶的表达会选择性增加。迄今未知的杂种LM是通过M亚基和L亚基的体外杂交产生的。
