Srivastava B I, Han T
FEBS Lett. 1984 May 7;170(1):152-6. doi: 10.1016/0014-5793(84)81389-7.
Several enzyme activities were examined to establish a relationship between their expression and terminal differentiation of B-chronic lymphocytic leukemia (CLL) cells to plasma cells by 12-O-tetradecanoylphorbol-13-acetate (TPA). Although adenosine deaminase activity did not change significantly, thymidine phosphorylase and purine nucleoside phosphorylase increased 2-3-fold on TPA-induced differentiation of CLL cells. In addition, cytochemical reactions for non-specific esterase and acid phosphatase changed from very weak to intense on differentiation of CLL cells to plasma cells. The above markers, particularly cytochemical, could be useful for the classification of B-cell malignancies and for studying B-cell differentiation.
检测了几种酶活性，以确定它们的表达与经12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导B - 慢性淋巴细胞白血病（CLL）细胞向浆细胞终末分化之间的关系。尽管腺苷脱氨酶活性没有显著变化，但胸苷磷酸化酶和嘌呤核苷磷酸化酶在TPA诱导CLL细胞分化时增加了2 - 3倍。此外，在CLL细胞向浆细胞分化过程中，非特异性酯酶和酸性磷酸酶的细胞化学反应从非常弱变为强烈。上述标志物，尤其是细胞化学标志物，可用于B细胞恶性肿瘤的分类以及研究B细胞分化。
