Dufer J, Bernard J
Leuk Res. 1984;8(5):813-20. doi: 10.1016/0145-2126(84)90102-4.
Four acid hydrolases, acid phosphatase (AP), alpha-naphthyl acetate acid esterase (ANAE), beta-glucuronidase (BG) and N-acetyl-beta-glucosaminidase (NABG) were determined cytochemically in B-chronic lymphocytic leukaemia (B-CLL) cells exposed in vitro to the tumor promoter 12-0-tetradecanoyl phorbol 13 acetate (TPA). TPA, which has been previously shown to induce B-CLL cells to mature towards plasmacytoid cells, results in the progressive expression of the enzymes tested in the cytoplasm of malignant cells, in particular AP and ANAE. Furthermore, the sensitivity to inhibitors and the pattern of reactivity of ANAE provide evidence for an enzyme subtype normally restricted to plasma cells. Thus, acid hydrolases--some of which showing plasma cell type of activity--are expressed during B-CLL cells differentiation induced in vitro. These results confirm the value of cytochemistry in subtyping B-cell malignancies.
采用细胞化学方法测定了体外暴露于肿瘤启动子12-O-十四烷酰佛波醇-13-乙酸酯（TPA）的B细胞慢性淋巴细胞白血病（B-CLL）细胞中的四种酸性水解酶，即酸性磷酸酶（AP）、α-萘乙酸酯酶（ANAE）、β-葡萄糖醛酸酶（BG）和N-乙酰-β-氨基葡萄糖苷酶（NABG）。TPA先前已被证明可诱导B-CLL细胞向浆细胞样细胞成熟，导致所检测的酶在恶性细胞的细胞质中逐渐表达，尤其是AP和ANAE。此外，ANAE对抑制剂的敏感性和反应模式为一种通常局限于浆细胞的酶亚型提供了证据。因此，酸性水解酶——其中一些表现出浆细胞类型的活性——在体外诱导的B-CLL细胞分化过程中表达。这些结果证实了细胞化学在B细胞恶性肿瘤亚型分类中的价值。
