Cahour A, Debeire P, Hartmann L, Montreuil J, van Halbeek H, Vliegenthart J F
FEBS Lett. 1984 May 21;170(2):343-9. doi: 10.1016/0014-5793(84)81341-1.
The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary structure of the major compounds was elucidated on the basis of carbohydrate analysis, methylation analysis, including mass-spectrometry, and 500 MHz 1H-NMR spectroscopy. For both patients, this appeared to be a monosialyl monofucosyl biantennary structure; the compounds differed by the presence of an intersecting N-acetylglucosamine residue.
来自两名华氏巨球蛋白血症患者的病理性人免疫球蛋白M的碳水化合物链通过肼解作用被释放出来。通过伴刀豆球蛋白A亲和层析获得N-乙酰乳糖胺型聚糖，并通过高压纸电泳进行分离。主要化合物的一级结构是基于碳水化合物分析、甲基化分析（包括质谱分析）以及500兆赫的1H-NMR光谱学确定的。对于两名患者来说，其似乎都是一种单唾液酸单岩藻糖基双天线结构；这些化合物因存在一个交叉的N-乙酰葡糖胺残基而有所不同。
