Debray H, Fournet B, Montreuil J, Dorland L, Vliegenthart F C
Eur J Biochem. 1981 Apr;115(3):559-63. doi: 10.1111/j.1432-1033.1981.tb06239.x.
Glycopeptides obtained by exhaustive pronase digestion of delipidated rat liver plasmic membranes were purified by gel filtration on Sephadex G-25. These glycopeptides were further fractionated by affinity chromatography on a concanavalin-A--Sepharose 4B column into the following fractions: (a) glycopeptides which did not bind to the column (fraction 1); (b) glycopeptides with weak affinity for concanavalin-A--Sepharose, which could be eluted with buffer only (fraction 2); (c) glycopeptides retained on the column and which could be eluted specifically with buffer containing 0.2 M methyl alpha-glucoside (fraction 3). On the basis of the carbohydrate composition, methylation analysis and 360-MHz 1H-NMR spectroscopy, the following primary structure of a glycan in fraction 2 is proposed: (see formula in text).
通过对脱脂大鼠肝质膜进行彻底的链霉蛋白酶消化获得的糖肽，经Sephadex G - 25凝胶过滤纯化。这些糖肽通过在伴刀豆球蛋白A - 琼脂糖4B柱上进行亲和色谱进一步分离成以下组分：(a) 不与柱结合的糖肽（组分1）；(b) 对伴刀豆球蛋白A - 琼脂糖亲和力较弱、仅能用缓冲液洗脱的糖肽（组分2）；(c) 保留在柱上且能用含0.2 Mα - 甲基葡萄糖苷的缓冲液特异性洗脱的糖肽（组分3）。基于碳水化合物组成、甲基化分析和360 - MHz 1H - NMR光谱，提出了组分2中一种聚糖的以下一级结构：（见文中公式）。
