Curtsinger J M, Fan D P
J Immunol. 1984 Jul;133(1):267-72.
The primary anti-H-2k allospecific cytolytic T lymphocyte (CTL) response by BALB/c (H-2d) spleen cells in vitro to x-irradiated RDM4 (H-2k) tumor cells is weak. This response has been shown to be augmented by CTL helper factor (CHF), a factor present in supernatants of spleen cells cultured with Sendai virus (SC-CM). Conditioned medium from WEHI-3 cells (WEHI-CM) also contains activity that augments the BALB/c anti- RDM4 CTL response. Attempts to separate the CHF activity from interleukin 3 (IL 3), also present in WEHI-CM, were unsuccessful. Purified IL 3 was then tested, and was found to increase the BALB/c anti- RDM4 CTL response by five- to 10-fold. IL 3 is apparently the only material in WEHI-CM that is active in this assay. The response is apparently a classical CTL response because: 1) the effector cells are sensitive to monoclonal anti-Thy-1.2 antibody plus C; 2) the response is dependent on antigen stimulation, and it peaks on day 5 or 6 of culture; and 3) the effector cells are specific for H-2k targets. IL 3 must be added very early during the in vitro culture period for maximal augmentation of the response, consistent with possible action of IL 3 as a differentiation factor.
在体外，BALB/c（H-2d）脾细胞对经X射线照射的RDM4（H-2k）肿瘤细胞产生的主要抗H-2k同种特异性细胞溶解T淋巴细胞（CTL）反应较弱。已证明，用仙台病毒培养的脾细胞上清液中存在的CTL辅助因子（CHF）可增强这种反应。来自WEHI-3细胞的条件培养基（WEHI-CM）也含有增强BALB/c抗RDM4 CTL反应的活性物质。将CHF活性与同样存在于WEHI-CM中的白细胞介素3（IL-3）分离的尝试未成功。随后对纯化的IL-3进行了测试，发现其可使BALB/c抗RDM4 CTL反应增强5至10倍。IL-3显然是WEHI-CM中在该检测中具有活性的唯一物质。该反应显然是一种典型的CTL反应，原因如下：1）效应细胞对单克隆抗Thy-1.2抗体加补体敏感；2）反应依赖于抗原刺激，且在培养的第5天或第6天达到峰值；3）效应细胞对H-2k靶标具有特异性。为使反应得到最大程度的增强，必须在体外培养期非常早期就添加IL-3，这与IL-3作为分化因子的可能作用一致。
