Weaver C M, Schmitt H A, Stuart M A, Mason A C, Meyer N R, Elliott J G
J Nutr. 1984 Jun;114(6):1035-41. doi: 10.1093/jn/114.6.1035.
Soybeans can be efficiently labeled with radiolabeled iron by supplying the iron via a nutrient culture medium as an iron salt or as a chelate. By using dual labeled iron and EDTA, it was determined that none of the chelator was transported to the shoots with the iron. Therefore, the use of chelated iron as the iron source in the nutrient medium should not affect assessments of bioavailability of iron from plants. Bioavailability (determined from whole-body retention curves of 59Fe in rats) of iron from defatted soy flour was relatively high and addition of vitamin C did not significantly enhance absorption of iron from defatted soy flour.
通过在营养培养基中以铁盐或螯合物的形式提供铁，可以用放射性标记的铁有效地标记大豆。通过使用双标记的铁和乙二胺四乙酸（EDTA），确定没有螯合剂与铁一起转运到地上部分。因此，在营养培养基中使用螯合铁作为铁源不应影响对植物中铁生物有效性的评估。脱脂大豆粉中铁的生物有效性（根据大鼠体内59Fe的全身保留曲线确定）相对较高，添加维生素C并没有显著提高脱脂大豆粉中铁的吸收。
