枯草芽孢杆菌营养细胞中的芽孢形成促进因子。

Sporulation promoting factor in vegetative cells of Bacillus subtilis.

作者信息

Hachisuka Y, Imura H, Nakata A, Hattori E

出版信息

Microbiol Immunol. 1983;27(12):1005-19. doi: 10.1111/j.1348-0421.1983.tb02935.x.

DOI:10.1111/j.1348-0421.1983.tb02935.x

Abstract

A simple experimental system for detection of sporulation promoting factors was presented. This system showed that there was a sporulation promoting factor in the vegetative cells of Bacillus subtilis cultivated on nutrient agar for 9 hr (at stage T0). The factor was partially purified from the sonicate of vegetative cells by ethanol fractionation, gel filtration, chromatography and preparative gel electrophoresis, and it was identified as manganese-containing protein.

摘要

提出了一种用于检测孢子形成促进因子的简单实验系统。该系统表明，在营养琼脂上培养9小时（T0阶段）的枯草芽孢杆菌营养细胞中存在一种孢子形成促进因子。通过乙醇分级分离、凝胶过滤、色谱法和制备性凝胶电泳从营养细胞的超声破碎物中对该因子进行了部分纯化，并将其鉴定为含锰蛋白。