Waldburger C, Gonzalez D, Chambliss G H
Department of Bacteriology, University of Wisconsin-Madison 53706.
J Bacteriol. 1993 Oct;175(19):6321-7. doi: 10.1128/jb.175.19.6321-6327.1993.
We report the existence and partial purification of sporulation factor, which stimulates sporulation of Bacillus subtilis at low cell density. Proline or arginine is required for stimulation under the conditions of our assay. Sporulation factor is a small heat-stable substance produced by the cells during exponential growth phase. It is required in small amounts and is resistant to various proteolytic agents. Several spo mutants were tested for the ability to produce functional sporulation factor. All of these mutants produce factor and do not sporulate in the presence of factor from wild-type cells. Sporulation factor is not involved in the induction of alpha-amylase synthesis at the initiation of sporulation.
我们报道了芽孢形成因子的存在及部分纯化,该因子在低细胞密度下刺激枯草芽孢杆菌形成芽孢。在我们的测定条件下,脯氨酸或精氨酸是刺激所必需的。芽孢形成因子是细胞在指数生长期产生的一种小的热稳定物质。它只需少量,且对各种蛋白水解剂有抗性。测试了几个spo突变体产生功能性芽孢形成因子的能力。所有这些突变体都产生因子,并且在存在野生型细胞的因子时不形成芽孢。芽孢形成因子在芽孢形成起始时不参与α-淀粉酶合成的诱导。