Bourguignon J P, Gérard A, Franchimont P
Neuroendocrinology. 1984 May;38(5):376-81. doi: 10.1159/000123920.
Male rats aged between 15 and 75 days were orchidectomized or only anesthetized. After various periods from 2 h to 42 days, 8 animals of both groups were sacrificed. Serum concentrations of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) as well as the hypothalamic luteinizing hormone-releasing hormone (LHRH) content were determined by radioimmunoassay. At the times studied within 15 days of castration, no significant change in hypothalamic LHRH content was observed in rats orchidectomized at 21 days of age. However, when studied 3-6 weeks after castration, those animals showed a reduced hypothalamic LHRH content. No differences were observed between anesthetized and orchidectomized rats aged 15, 18 or 21 days and studied 1 week later. In contrast, castration of 75-day-old rats resulted in a significant reduction of the hypothalamic LHRH content after 2-15 days. A significant decrease was also observed 1 week after orchidectomy of rats aged 24-50 days. 1 day after orchidectomy, LH and FSH serum levels were markedly increased in 21- as well as 75-day-old rats. In the latter, serum LH concentrations did not change any further whereas in the former a plateau was only seen after 1 week. In rats orchidectomized at various ages ranging from 15 to 50 days, no consistent differences appeared in serum gonadotropin concentrations evaluated 7 days later. According to these data, variations in hypothalamic LHRH content after orchidectomy differ according to age and maturity. In 15- to 21-day-old rats, the age-related increase in hypothalamic LHRH content was not immediately affected by castration as it was in older animals.(ABSTRACT TRUNCATED AT 250 WORDS)
选取15至75日龄的雄性大鼠，对其进行睾丸切除或仅实施麻醉。在术后2小时至42天的不同时间段，每组各处死8只动物。采用放射免疫分析法测定血清中促黄体生成素（LH）和促卵泡生成素（FSH）的浓度以及下丘脑促黄体生成素释放激素（LHRH）的含量。在阉割后15天内进行研究时，21日龄阉割大鼠的下丘脑LHRH含量未观察到显著变化。然而，在阉割后3至6周进行研究时，这些动物的下丘脑LHRH含量降低。对15、18或21日龄的麻醉大鼠和睾丸切除大鼠在1周后进行研究，未观察到差异。相比之下，75日龄大鼠在阉割后2至15天，下丘脑LHRH含量显著降低。24至50日龄大鼠在睾丸切除1周后也观察到显著下降。睾丸切除1天后，21日龄和75日龄大鼠的血清LH和FSH水平均显著升高。对于75日龄大鼠，血清LH浓度不再进一步变化，而对于21日龄大鼠，仅在1周后出现平台期。在15至50日龄不同年龄段进行睾丸切除的大鼠中，7天后评估的血清促性腺激素浓度未出现一致差异。根据这些数据，睾丸切除后下丘脑LHRH含量的变化因年龄和成熟度而异。在15至21日龄的大鼠中，与年龄相关的下丘脑LHRH含量增加不像在年长动物中那样立即受到阉割的影响。（摘要截选至250词）
