Hadler W A, Silveira S R
Acta Histochem. 1984;74(1):25-32. doi: 10.1016/S0065-1281(84)80021-5.
The peracetic acid-toluidine blue and the peracetic acid-Schiff reactions as well as the failure of HgCl2-formalin fixative for inhibiting these reactions appears as a very useful method for free cholesterol histochemical detection and to discriminate it from its esters and from some of its metabolic products such as 7-dehydrocholesterol and vitamins D. The cholesterol histochemical detection provided by those reactions appears specific taking into account the findings afforded by spot tests. The peracetic acid-toluidine blue reaction is very suitable for histochemical purposes on tissue sections, since it does not produces tissues damages, it is sensitive and the stained end product is almost insoluble in the solvents frequently used in histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-histological techniques. The previous ribonuclease treatment and methylation are very useful in avoiding the basophil substances interference on the peracetic acid-toluidine blue reaction. The 2,4-dinitrophenylhydrazine carbonyl group blockade is useful to decrease the influence of carbonyl group on the peracetic acid-Schiff reaction. The peracetic acid-toluidine blue reaction is more sensitive and more specific than peracetic acid-Schiff reaction, since the tissues do contain Schiff reactive products unable to be 2,4-dinitrophenylhydrazine blocked which interfere on the results of the latter reaction decreasing its specificity and its sensitivity. The cholesterol histochemical detection based on the peracetic acid-toluidine blue and peracetic acid-Schiff reaction as it is suitable in discriminating free cholesterol from its esters as well as from some of its metabolic products it appears very useful for metabolic studies on tissue sections.
过氧乙酸 - 甲苯胺蓝反应、过氧乙酸 - 席夫反应以及氯化汞 - 甲醛固定剂无法抑制这些反应，这似乎是一种非常有用的游离胆固醇组织化学检测方法，可将其与胆固醇酯及其一些代谢产物（如7 - 脱氢胆固醇和维生素D）区分开来。考虑到斑点试验的结果，这些反应所提供的胆固醇组织化学检测似乎具有特异性。过氧乙酸 - 甲苯胺蓝反应非常适合用于组织切片的组织化学目的，因为它不会对组织造成损伤，具有敏感性，且染色终产物几乎不溶于组织学技术中常用的溶剂。先前的核糖核酸酶处理和甲基化对于避免嗜碱性物质对过氧乙酸 - 甲苯胺蓝反应的干扰非常有用。2,4 - 二硝基苯肼羰基阻断有助于减少羰基对过氧乙酸 - 组织学技术的影响。先前的核糖核酸酶处理和甲基化对于避免嗜碱性物质对过氧乙酸 - 甲苯胺蓝反应的干扰非常有用。2,4 - 二硝基苯肼羰基阻断有助于减少羰基对过氧乙酸 - 席夫反应的影响。过氧乙酸 - 甲苯胺蓝反应比过氧乙酸 - 席夫反应更敏感、更具特异性，因为组织中确实含有无法被2,4 - 二硝基苯肼阻断的席夫反应性产物，这些产物会干扰后者反应的结果，降低其特异性和敏感性。基于过氧乙酸 - 甲苯胺蓝和过氧乙酸 - 席夫反应的胆固醇组织化学检测，由于其适用于将游离胆固醇与其酯类以及一些代谢产物区分开来，对于组织切片的代谢研究似乎非常有用。
