通过酶测定法分析商业花粉提取物。II. 测定花粉提取物中植物物质的污染情况。
Analysis of commercial pollen extracts by enzyme determination. II. Determination of the contamination of pollen extracts with plant matter.
作者信息
Bousquet J, Guerin B, Hewitt B, Cour P, Michel F
出版信息
Allergol Immunopathol (Madr). 1984 Jan-Feb;12(1):11-8.
The contamination of pollen extracts by plant matter was studied by means of enzymes. Beta-glucosidase was detected only in flowers and leaves by using the Api-Zym System. 29 pollen extracts were prepared after microscopic control. Among these 23 did not contain any plant matter and no beta-glucosidase activity could be detected. In contrast, 6 extracts were contaminated with plant matter and in every case beta-glucosidase activity was found 34 commercial pollen extracts completed the study. beta-glucosidase activity was present in 8 of them. It may be postulated that glucosidase activity detected using 2- naphthyl - beta D-glucopyranoside as a substrate, can be correlated with the presence of plant matter from the raw material from which the extract was prepared. This contamination may be important to determine since some additional sensitization may occur with such extracts during immunotherapy.
通过酶法研究了花粉提取物被植物物质污染的情况。使用Api-Zym系统仅在花朵和叶子中检测到β-葡萄糖苷酶。在显微镜控制后制备了29份花粉提取物。其中23份不含任何植物物质，未检测到β-葡萄糖苷酶活性。相比之下，6份提取物被植物物质污染，并且在每种情况下都发现了β-葡萄糖苷酶活性。34份市售花粉提取物完成了该研究。其中8份存在β-葡萄糖苷酶活性。可以推测，以2-萘基-β-D-吡喃葡萄糖苷为底物检测到的葡萄糖苷酶活性可能与制备提取物的原材料中植物物质的存在相关。确定这种污染可能很重要，因为在免疫治疗期间，此类提取物可能会引起一些额外的致敏反应。
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