Yang C Y, Wakil S J
Anal Biochem. 1984 Feb;137(1):54-7. doi: 10.1016/0003-2697(84)90345-2.
The separation of all common dimethylaminoazobenzenethiohydantoin (DABTH) amino acids derived from modified Edman sequencing can be achieved by using high-performance liquid chromatography. All derivatives, including DABTH-Ile and DABTH-Leu, can be readily separated in a solvent mixture of sodium acetate buffer and 1% ethylene dichloride in acetonitrile. The high absorbance of the DABTH amino acids at 436 nm makes possible the quantitative determination of these derivatives at picomole concentrations in a relatively short time (30-40 min).
通过高效液相色谱法可以实现对源自改良埃德曼测序的所有常见二甲基氨基偶氮苯硫代乙内酰脲（DABTH）氨基酸的分离。所有衍生物，包括DABTH - 异亮氨酸和DABTH - 亮氨酸，都能在乙酸钠缓冲液与乙腈中1%二氯乙烷的混合溶剂中轻松分离。DABTH氨基酸在436nm处的高吸光度使得在相对较短的时间内（30 - 40分钟）对皮摩尔浓度的这些衍生物进行定量测定成为可能。
