Shrago E, Spenneta T, Shahidsaless F M, Elson C
Metabolism. 1978 May;27(5):563-9. doi: 10.1016/0026-0495(78)90023-9.
While both pyruvate and lactate are good substrates for glyceride-glycerol synthesis in isolated adipocytes from fed rats and guinea pigs, neither alanine nor serine appear to support glyceroneogenesis. Fasting increases the proportion of radioactive pyruvate or lactate incorporated into glyceride-glycerol and reciprocally decreases the proportion incorporated into fatty acids. However, the total incorporation of radioactivity into triacylglycerol is considerably lower in isolated adipocytes from fasted than from fed animals. Addition of glucose to the incubation medium promotes the incorporation of radioactive lactate into both fatty acids and glyceride-glycerol by adipocytes from fasted as well as fed animals. The concentration of alpha-glycerolphosphate is considerably higher in adipose tissue of fed than fasted animals. In general, these results support the presence of a glyceroneogenic pathway in rat and guinea pig adipose tissue. However, it would appear that the physiologic significance of this pathway is less important in the fasted than the fed state, where it may play some role in the esterification of intracellular fatty acids.
对于喂食后的大鼠和豚鼠分离出的脂肪细胞而言，丙酮酸和乳酸都是甘油酯 - 甘油合成的良好底物，但丙氨酸和丝氨酸似乎都不能支持甘油异生作用。禁食会增加掺入甘油酯 - 甘油中的放射性丙酮酸或乳酸的比例，相应地降低掺入脂肪酸中的比例。然而，禁食动物分离出的脂肪细胞中，放射性物质掺入三酰甘油的总量比喂食动物的要低得多。向孵育培养基中添加葡萄糖可促进禁食和喂食动物的脂肪细胞将放射性乳酸掺入脂肪酸和甘油酯 - 甘油中。喂食动物脂肪组织中α - 甘油磷酸的浓度比禁食动物的高得多。总体而言，这些结果支持大鼠和豚鼠脂肪组织中存在甘油异生途径。然而，似乎该途径在禁食状态下的生理意义不如在喂食状态下重要，在喂食状态下它可能在细胞内脂肪酸的酯化中发挥一定作用。
