Kim I, Greenwald G S
Biol Reprod. 1984 Jun;30(5):1063-72. doi: 10.1095/biolreprod30.5.1063.
The amounts of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin (Prl) required for follicular development or luteal maintenance were established by hypophysectomizing hamsters between 0900-1100 h of Day 1 of the cycle (=morning of ovulation) and injecting the animals s.c. over the next 4 days to stimulate the normal cycle. Animals were killed at 1300 h on Day 4 or injected with human chorionic gonadotropin (hCG) at 1500 h on Day 4 and killed the next morning to determine the ovulation rate. Daily injection of 20 IU hCG resulted in 8 antral follicles per ovary and ovulation of 12.4 ova, but 20 micrograms LH (in saline) failed to develop any antral follicles; 20 micrograms LH in polyvinylpyrrolidone (PVP) - which prolongs its biological activity - matured 2-4 antral follicles per ovary and the animals ovulated 3 ova in response to hCG. However, injection of 5 micrograms FSH on Day 1 plus 20 micrograms LH (in PVP) on Days 1-4 produced normal numbers of antral follicles and ovulation of 15 ova. When 5 micrograms FSH was injected daily along with LH (in PVP), follicular development was accentuated and the animals ovulated 49 ova. Keeping FSH constant at 5 micrograms/day and reducing LH from 20 micrograms to 10 or 5 micrograms per day did not reduce the number of follicles maturing or ovulating. Daily injection of Prl did not affect follicular development but maintained corpora lutea (CL) in some animals. Combined injection of Prl and LH (in PVP), but not FSH, maintained active CL in all animals. Thus, the hypophysectomized hamster requires tonically high levels of LH for the hormone to exert its follicular and luteal effects with FSH and Prl, respectively.
通过在周期第1天的0900 - 1100时（即排卵日上午）切除仓鼠垂体，并在接下来4天对动物进行皮下注射以刺激正常周期，来确定卵泡发育或黄体维持所需的促黄体生成素（LH）、促卵泡生成素（FSH）和催乳素（Prl）的量。在第4天的1300时处死动物，或在第4天的1500时注射人绒毛膜促性腺激素（hCG），并于次日早晨处死以确定排卵率。每日注射20 IU hCG可使每个卵巢有8个窦状卵泡，排卵12.4个卵子，但20微克LH（溶于生理盐水）未能使任何窦状卵泡发育；溶于聚乙烯吡咯烷酮（PVP）中的20微克LH（可延长其生物活性）使每个卵巢有2 - 4个窦状卵泡成熟，并且动物在注射hCG后排卵3个卵子。然而，在第1天注射5微克FSH并在第1 - 4天注射20微克LH（溶于PVP）可产生正常数量的窦状卵泡，排卵15个卵子。当每天注射5微克FSH并同时注射LH（溶于PVP）时，卵泡发育加速，动物排卵49个卵子。将FSH保持在每天5微克不变，同时将LH从每天20微克降至10或5微克，并不会减少成熟或排卵的卵泡数量。每日注射Prl不影响卵泡发育，但可使一些动物的黄体维持。联合注射Prl和LH（溶于PVP）而非FSH，可使所有动物的黄体保持活性。因此，切除垂体的仓鼠需要持续高水平的LH，该激素才能分别与FSH和Prl共同发挥其卵泡和黄体效应。
