Seufert C D, Mewes W, Soeling H D
Eur J Clin Invest. 1984 Apr;14(2):163-70. doi: 10.1111/j.1365-2362.1984.tb02107.x.
The turnover of ketone bodies and acetate was evaluated as well from the disappearance rate of (3-14C)acetoacetate or (1-14C)acetate respectively as from the conversion of FFA into these metabolites in normal weight and obese overnight-fasted and in obese long-term starved patients. The disappearance rate of (1-14C)oleate was the same in all three groups. Long-term starvation enhanced ketone body turn-over almost 10-fold, whereas the disappearance rate for ketone bodies decreased from 0.035 to 0.015 min-1. Under the same circumstances the turnover of acetate was about 1 mumol g-1 min-1 accounting for about 5% of FFA turnover. Long-term starvation decreased the conversion of (1-14C)oleate into triglycerides by almost 50% and increased the (2-C)-(4-C)/(1-C) ratio of radioactivity in ketone bodies. The reincorporation of radioactivity from the (1-C)position of (1-14C)oleate into the ( (2-C)-(n-C) ) position of FFA, which is a measure of the reutilization of acetyl-CoA for FFA synthesis decreased significantly during long-term starvation.
通过分别测定（3-14C）乙酰乙酸盐或（1-14C）乙酸盐的消失率以及游离脂肪酸（FFA）向这些代谢物的转化情况，对正常体重、过夜禁食的肥胖患者以及长期饥饿的肥胖患者体内酮体和乙酸盐的周转率进行了评估。三组中（1-14C）油酸的消失率相同。长期饥饿使酮体周转率提高了近10倍，而酮体的消失率从0.035降至0.015分钟-1。在相同情况下，乙酸盐的周转率约为1微摩尔克-1分钟-1，约占FFA周转率的5%。长期饥饿使（1-14C）油酸向甘油三酯的转化降低了近50%，并增加了酮体中放射性的（2-C）-（4-C）/（1-C）比率。长期饥饿期间，（1-14C）油酸（1-C）位置的放射性重新掺入FFA（（2-C）-（n-C））位置的情况显著减少，这是乙酰辅酶A用于FFA合成的再利用程度的一种衡量指标。
