Lundsgaard T
Intervirology. 1984;22(1):50-5. doi: 10.1159/000149533.
Leaf tissue from plants infected with Festuca leaf streak virus was extracted with a buffer containing a nonionic detergent; this treatment released the G protein from the nucleocapsid of virions. The extract was tested with antiserum by double immunodiffusion for G protein and by immunosorbent electron microscopy with 'decoration' for nucleocapsid antigens. Clear homologous reactions were obtained. No heterologous reactions were observed with similar preparations of barley yellow striate mosaic, northern cereal mosaic, and cereal chlorotic mottle viruses.
用含有非离子去污剂的缓冲液提取感染了羊茅叶条斑病毒的植物叶片组织；这种处理从病毒粒子的核衣壳中释放出G蛋白。提取物通过双免疫扩散法用抗血清检测G蛋白，并通过“装饰”免疫吸附电子显微镜检测核衣壳抗原。获得了明显的同源反应。用大麦黄条纹花叶病毒、北方谷物花叶病毒和谷物褪绿斑驳病毒的类似制剂未观察到异源反应。
