Ojo-Amaize E A, Vilcek J, Cochrane A H, Nussenzweig R S
J Immunol. 1984 Aug;133(2):1005-9.
Enhanced natural killer (NK) activity was detected in the spleens of mice as early as 24 hr after single i.v. inoculation with gamma-irradiated Plasmodium berghei sporozoites. The activity peaked at 48 hr post-injection, and declined below baseline level by day 8. Reinoculation of mice with irradiated sporozoites produced an increased NK activity significantly smaller than the original activity. Spleen cells sensitized in vivo as well as nonsensitized spleen cells stimulated in vitro with sporozoites produced high levels of interferon (IFN) and displayed enhanced NK activity. Characterization of the IFN through the use of specific antibodies revealed that it was mainly IFN-gamma. The cellular basis for IFN-gamma induction was linked to the mitogenicity of P. berghei sporozoites for T cells. The possibility exists that IFN-gamma may have a regulatory effect on antibody production against P. berghei sporozoites.
早在单次静脉注射经γ射线照射的伯氏疟原虫子孢子24小时后，就检测到小鼠脾脏中自然杀伤（NK）活性增强。该活性在注射后48小时达到峰值，并在第8天降至基线水平以下。用经照射的子孢子再次接种小鼠，产生的NK活性增加明显小于原始活性。体内致敏的脾细胞以及体外经子孢子刺激的未致敏脾细胞产生高水平的干扰素（IFN），并表现出增强的NK活性。通过使用特异性抗体对IFN进行表征，发现其主要为IFN-γ。IFN-γ诱导的细胞基础与伯氏疟原虫子孢子对T细胞的促有丝分裂作用有关。IFN-γ可能对针对伯氏疟原虫子孢子的抗体产生具有调节作用。
