Macchia D D, Didelot M J, Anderson W M
Muscle Nerve. 1984 Jun;7(5):415-23. doi: 10.1002/mus.880070512.
Isolated semitendinosus muscles of the toad were incubated in both Ringer solution and toad plasma for periods ranging from 45 to 170 minutes. The extracellular volume (ECV), as measured from the equilibrium distribution of 35SO4(2-), was found to increase (in gram extracellular (EC) water/gram wet weight) from 0.15 +/- 0.01 to 0.20 +/- 0.02 in muscles incubated in Ringer solution, whereas the ECV was found to remain constant in tissues incubated in plasma (0.16 +/- 0.01 to 0.17 +/- 0.01), for periods up to 170 minutes. Since the true ECV, as measured morphometrically, was found to remain unchanged for muscles incubated in Ringer solution for up to 3 hours, the data for these muscles were interpreted to represent a change in anion permeability of the cellular membranes in response to the absence of some plasma fraction from the Ringer solution (with an electrolyte composition and pH similar to that of plasma). When toad plasma albumin was added to the Ringer solution, in physiological concentrations, the ECV of the muscles was observed to remain relatively constant during the 3-hour incubation period. This albumin effect on anion permeability was found to be albumin concentration-dependent. Further, other sources of albumin were found to have varying degrees of effect on the anion permeability of toad muscle, with bovine albumin serving as the most acceptable substitute for toad albumin, when incubating semitendinosus muscles.
将蟾蜍的分离半腱肌在林格氏液和蟾蜍血浆中孵育45至170分钟。通过35SO4(2-)的平衡分布测得的细胞外液量（ECV）（以克细胞外（EC）水/克湿重计），在林格氏液中孵育的肌肉中从0.15±0.01增加到0.20±0.02，而在血浆中孵育的组织中，ECV在长达170分钟的时间内保持恒定（从0.16±0.01到0.17±0.01）。由于通过形态计量学测量发现，在林格氏液中孵育长达3小时的肌肉的真实ECV保持不变，因此这些肌肉的数据被解释为细胞膜阴离子通透性的变化，这是对林格氏液中某些血浆成分缺失的反应（其电解质组成和pH与血浆相似）。当将生理浓度的蟾蜍血浆白蛋白添加到林格氏液中时，观察到肌肉的ECV在3小时的孵育期内保持相对恒定。发现这种白蛋白对阴离子通透性的影响是白蛋白浓度依赖性的。此外，还发现其他来源的白蛋白对蟾蜍肌肉的阴离子通透性有不同程度的影响，在孵育半腱肌时，牛白蛋白是蟾蜍白蛋白最可接受的替代品。
