Macchia D D, Page E, Polimeni P I
Am J Physiol. 1979 Sep;237(3):C125-30. doi: 10.1152/ajpcell.1979.237.3.C125.
The distributions of a charged and an uncharged extracellular tracer in the interstitial spaces of skeletal and heart muscles were examined in vivo by a double-labeling technique. 35SO4(2-) and [3H]sucrose were simultaneously injected intraperitoneally into rats and toads, and extracellular volume was determined in the rat gastrocnemius and left ventricle and in the toad semitendinosus. In nephrectomized rats and in toads with intact kidneys, sucrose and SO4(2-) spaces were constant for several hours. Sucrose and SO4(2-) spaces did not significantly differ in rat ventricle (P greater than 0.80); in rat gastrocnemius the sucrose space was much larger than SO4(2-) space (2P less than 0.0005), while in toad semitendinosus sucrose space was somewhat smaller than SO4(2-) space (2P less than 0.005). These observations suggest that fixed charges in the interstitial compartment can lead to extracellular anion exclusion in some tissues and perhaps to accumulation in others. The magnitude and direction of these effects differ for different striated muscles.
采用双标记技术在活体状态下研究了带电荷和不带电荷的细胞外示踪剂在骨骼肌和心肌间质中的分布情况。将35SO4(2-)和[3H]蔗糖同时腹腔注射到大鼠和蟾蜍体内，并测定大鼠腓肠肌、左心室以及蟾蜍半腱肌的细胞外液量。在肾切除的大鼠和肾脏完好的蟾蜍中，蔗糖和SO4(2-)的分布空间在数小时内保持恒定。大鼠心室中蔗糖和SO4(2-)的分布空间无显著差异（P>0.80）；在大鼠腓肠肌中，蔗糖分布空间远大于SO4(2-)的分布空间（2P<0.0005），而在蟾蜍半腱肌中，蔗糖分布空间略小于SO4(2-)的分布空间（2P<0.005）。这些观察结果表明，间质中的固定电荷可能导致某些组织中细胞外阴离子的排除，而在其他组织中可能导致阴离子的蓄积。这些效应的大小和方向在不同的横纹肌中有所不同。
