Tepe S J, Zenick H
Toxicology. 1984 Jul;32(1):47-56. doi: 10.1016/0300-483x(84)90033-7.
Two experimental protocols were employed to determine the effects of carbon disulfide (CS2) on the reproductive system of the male rat. In the first experiment, adult Long Evans hooded rats were exposed to 0, 350 or 600 ppm CS2 vapor for 10 weeks (5 h/day, 5 days/week). CS2 exposure caused no change in reproductive organ weights nor in plasma gonadotropin levels. However, animals exposed to 600 ppm CS2 had slightly lower epididymal sperm counts and significantly reduced plasma testosterone levels. In order to determine if monitoring hormone levels and sperm status in the same male over time might increase the sensitivity of detecting a toxic reaction, the second protocol was employed. Male rats were exposed to 0 or 600 ppm CS2. After 0, 1, 4, 7 and 10 weeks of exposure, males were observed for mating behavior, and ejaculated sperm count and plasma hormone levels were determined. Animals exposed to 600 ppm CS2 had significantly shorter times to mount and to ejaculate and decreased ejaculated sperm counts. Plasma gonadotropin levels were similar in both groups while plasma testosterone levels were marginally depressed in CS2-exposed animals in the early weeks. These data indicate that CS2 is a toxin of the male reproductive system resulting in abnormal coital behavior and decreased sperm counts. The second experimental protocol proves to be a sensitive method for assessing adverse effects in the male reproductive system.
采用两种实验方案来确定二硫化碳（CS2）对雄性大鼠生殖系统的影响。在第一个实验中，成年Long Evans有帽大鼠暴露于0、350或600 ppm的CS2蒸气中10周（每天5小时，每周5天）。接触CS2并未导致生殖器官重量或血浆促性腺激素水平发生变化。然而，暴露于600 ppm CS2的动物附睾精子计数略低，血浆睾酮水平显著降低。为了确定随时间监测同一雄性动物的激素水平和精子状态是否可能提高检测毒性反应的敏感性，采用了第二个方案。雄性大鼠暴露于0或600 ppm CS2。在暴露0、1、4、7和10周后，观察雄性大鼠的交配行为，并测定射精精子计数和血浆激素水平。暴露于600 ppm CS2的动物爬跨和射精时间显著缩短，射精精子计数减少。两组血浆促性腺激素水平相似，而在早期，暴露于CS2的动物血浆睾酮水平略有下降。这些数据表明，CS2是一种雄性生殖系统毒素，会导致异常交配行为和精子计数减少。第二个实验方案被证明是评估雄性生殖系统不良反应的一种敏感方法。
