Zenick H, Blackburn K, Hope E, Baldwin D
Toxicol Appl Pharmacol. 1984 Apr;73(2):275-83. doi: 10.1016/0041-008x(84)90333-8.
The present study was undertaken to evaluate the endocrinologic and spermatogenic effects of carbon disulfide (CS2) exposure in the rat. Adult, male rats were exposed to either 600 ppm CS2 or filtered air for 6 hr/day for 5 days/week for 10 weeks. One week prior to exposure and then at Weeks 1, 4, 7, and 10, males were placed with ovariectomized, hormonally primed females, and copulatory behaviors were scored. Fifteen minutes postcopulation, the female was killed and the ejaculate was recovered from the excised uterine tract along with the semen plug. Sperm counts, sperm motility, and morphology were determined. A blood sample was obtained for analyses of testosterone, follicle-stimulating (FSH), and luteinizing hormone (LH). At the end of the 10th week, five animals in each group were challenged with either human chorionic gonadotropin (HCG, 50 IU/animal, iv) or gonadotropin-releasing hormone (GnRH, 100 ng/animal, iv), and the testosterone or gonadotropin responses were monitored over time. Animals were subsequently killed with one epididymis and testis processed for histology and a sperm count determined from the other epididymis. Analysis revealed that CS2 exposure produced significant alterations in copulatory behavior and a decrease in ejaculated sperm counts by the fourth and seventh weeks of exposure, respectively. No endocrinologic alterations were observed. Moreover, caudal epididymal sperm counts were not depressed and the testes appeared histologically normal. These data suggest that CS2 does not exert a direct effect on the testes, but rather may interfere with the processes regulating sperm transport and ejaculation.
本研究旨在评估大鼠接触二硫化碳（CS2）后的内分泌和生精作用。成年雄性大鼠每天暴露于600 ppm CS2或过滤空气中6小时，每周5天，共10周。在暴露前一周以及第1、4、7和10周，将雄性大鼠与去卵巢且经激素预处理的雌性大鼠放在一起，并对交配行为进行评分。交配后15分钟，处死雌性大鼠，从切除的子宫中回收射精物及精液栓。测定精子数量、精子活力和形态。采集血样分析睾酮、促卵泡激素（FSH）和促黄体生成素（LH）。在第10周结束时，每组5只动物分别接受人绒毛膜促性腺激素（HCG，50 IU/只，静脉注射）或促性腺激素释放激素（GnRH，100 ng/只，静脉注射）刺激，并随时间监测睾酮或促性腺激素反应。随后处死动物，一侧附睾和睾丸用于组织学检查，另一侧附睾用于测定精子数量。分析显示，接触CS2分别在接触的第4周和第7周导致交配行为发生显著改变以及射精精子数量减少。未观察到内分泌改变。此外，附睾尾部精子数量未减少，睾丸组织学检查显示正常。这些数据表明，CS2对睾丸没有直接作用，而是可能干扰调节精子运输和射精的过程。
