Fernández-Pardal J, Granata A R, Barrio A, Gimeno A L
Acta Physiol Lat Am. 1983;33(4):293-8.
The release of 3H-catecholamines evoked by Black Widow spider venom gland extract (BW-GE) has been studied in the isolated rat hypothalamus (HT), occipital cortex (OC), preloaded with 3H-noradrenaline, and isolated caudate nucleus (CN) preloaded with 3H-dopamine. The BWGE at a concentration of 0.04 gland/ml increased significantly 3H-output in isolated slices of rat HT, OC and CN. This effect was markedly depressed when control calcium concentration in the medium (1.68 mM) was reduced (0-0.56 mM) or enhanced (3 mM), as well as in the presence of an organic Ca2+ antagonist, verapamil (10 microM), or ionophore A 23187 (40 microM), a substance that increases the influx of calcium into the cell. Morphine (up to 0.4 mM) evoked no effect upon 3H-noradrenaline release induced by BWGE. Morphine (10 microM), but not ionophore A 23187 or high Ca2+ (3 mM), reduced 3H-noradrenaline release induced by 20 mM K+. Low Ca2+ and verapamil produced similar effects than those observed for BWGE. Our results demonstrate differences between BWGE and potassium stimuli, and indicate that BWGE releases 3H-catecholamines by a calcium dependent process.
在预先装载了3H-去甲肾上腺素的离体大鼠下丘脑（HT）、枕叶皮质（OC）以及预先装载了3H-多巴胺的离体尾状核（CN）中，研究了黑寡妇蜘蛛毒液腺提取物（BW-GE）诱发的3H-儿茶酚胺释放。浓度为0.04腺体/毫升的BWGE显著增加了大鼠HT、OC和CN离体切片中的3H释放量。当培养基中的对照钙浓度（1.68毫摩尔/升）降低（0 - 0.56毫摩尔/升）或升高（3毫摩尔/升）时，以及在存在有机钙拮抗剂维拉帕米（10微摩尔）或离子载体A 23187（40微摩尔，一种增加钙流入细胞的物质）的情况下，这种效应明显受到抑制。吗啡（高达0.4毫摩尔）对BWGE诱导的3H-去甲肾上腺素释放没有影响。吗啡（10微摩尔），但不是离子载体A 23187或高钙（3毫摩尔），减少了由20毫摩尔钾诱导的3H-去甲肾上腺素释放。低钙和维拉帕米产生的效应与BWGE观察到的效应相似。我们的结果证明了BWGE和钾刺激之间的差异，并表明BWGE通过钙依赖过程释放3H-儿茶酚胺。
