Pardal J F, Granata A R, Barrio A, Gimeno A L
Naunyn Schmiedebergs Arch Pharmacol. 1980 Aug;313(1):27-31. doi: 10.1007/BF00505801.
In the isolated rat hypothalamus preincubated with 3H-noradrenaline, an extract from the venom glands of Latrodectus mactans elicited on overflow of tritium which consisted of 70--71% 3H-noradrenaline and 18% 3H-3,4-dihydroxyphenylglycol. Exposure to cocaine or pargyline diminished the formation of 3H-3,4-dihydroxyphenylglycol, suggesting that the glycol is formed presynaptically after neuronal reuptake of the transmitter released by the venom. Our data suggest that the venom depolarizes the noradrenergic neurones of rat hypothalamus, and that the depolarization leads to calcium-dependent release of 3H-noradrenaline.
在与3H-去甲肾上腺素预孵育的离体大鼠下丘脑标本中，黑寡妇蜘蛛毒腺提取物引发了氚的溢出，其中70 - 71%为3H-去甲肾上腺素，18%为3H-3,4-二羟基苯乙二醇。暴露于可卡因或帕吉林会减少3H-3,4-二羟基苯乙二醇的形成，这表明该二醇是在毒液释放的递质被神经元重新摄取后于突触前形成的。我们的数据表明，该毒液使大鼠下丘脑的去甲肾上腺素能神经元去极化，且这种去极化导致了3H-去甲肾上腺素的钙依赖性释放。
