Kondo T, Taniguchi N, Hirano T, Kawakami Y
Ann N Y Acad Sci. 1984;429:302-5. doi: 10.1111/j.1749-6632.1984.tb12353.x.
An inactive form of erythrocyte CA I was found in patients with primary aldosteronism. The levels of CA I in the patients were within the normal range: however, the specific esterase activity of CA I from three patients was in the range of 0.12-0.26 u/mg CA I (normal control: 0.66 +/- 0.1 u/mg CA I). In one subject, who had been treated with spironolactone, the specific activity of CA I was in the normal range. However after the termination of the drug the specific activity of CA I decreased to 0.3 u/mg CA I. Two months after adrenalectomy, the specific activity of the subjects was restored. The inactive CA I isolated from a patient was not immunologically distinguishable from that of normal individuals. The level of zinc in the inactive CA I was not different from that of normal CA I. The inhibitory effect of acetazolamide on the inactive CA I was weaker than that on normal CA I. The binding affinity of 3[H]acetazolamide to the inactive CA I was also lower than that of normal CA I. These results suggested the presence of a novel variant form of CA I in this disease.
在原发性醛固酮增多症患者中发现了红细胞碳酸酐酶I（CA I）的无活性形式。患者体内CA I的水平在正常范围内：然而，三名患者的CA I的特异性酯酶活性在0.12 - 0.26单位/毫克CA I范围内（正常对照：0.66±0.1单位/毫克CA I）。在一名接受螺内酯治疗的患者中，CA I的比活性在正常范围内。然而，停药后CA I的比活性降至0.3单位/毫克CA I。肾上腺切除术后两个月，患者的比活性恢复。从一名患者中分离出的无活性CA I在免疫方面与正常个体的无活性CA I无法区分。无活性CA I中的锌含量与正常CA I中的锌含量没有差异。乙酰唑胺对无活性CA I的抑制作用比对正常CA I的抑制作用弱。3[H]乙酰唑胺与无活性CA I的结合亲和力也低于正常CA I。这些结果表明该疾病中存在一种新型的CA I变异形式。
