Kondo T, Taniguchi N, Murao M, Takakuwa E
Clin Chim Acta. 1975 May 1;60(3):347-53. doi: 10.1016/0009-8981(75)90077-7.
Human erythrocyte carbonic anhydrase isozyme B was measured by a specific and immunological method. The levels of carbonic anhydrase B were determined in normal subjects, patients with hyperthyroidism, and patients with chronic obstructive lung disease and patients with epilepsy under treatment with acetazolamide, using the rapid assay method of single radial immunodiffusion. The levels of carbonic anhydrase B showed a decrease in hyperthyroidism, and increased in chronic obstructive lung diseases and epilepsies. Closely negative correlations were observed between carbonic anhydrase B and T3 resin sponge uptake or protein bound iodine (PBI) levels in hyperthyroidism. Simultaneously, carbonic anhydrase B-dependent esterase activity (active carbonic anhydrase B enzyme) was determined kinetically using the immunoadsorbent method in the above-mentioned patients. The results were compared with the total enzyme protein (active and inactive carbonic anhydrase B enzyme), estimated by the single radial immunodiffusion technique. In patients treated with acetazolamide, the "true" specific activity of the carbonic anhydrase B (carbonic anhydrase B-dependent esterase activity/total carbonic anhydrase B protein) decreased remarkably. In chronic obstructive lung disease, no remarkable changes were observed. On the other hand the specific activity was elevated in hyperthyroidism to 2 times higher than that of normal subjects. The clinical significance of these active enzymes is discussed.
采用一种特异性免疫方法测定人红细胞碳酸酐酶同工酶B。运用单扩散免疫比浊快速检测法，测定了正常受试者、甲状腺功能亢进患者、慢性阻塞性肺疾病患者以及接受乙酰唑胺治疗的癫痫患者体内碳酸酐酶B的水平。结果显示，甲状腺功能亢进患者体内碳酸酐酶B水平降低，慢性阻塞性肺疾病患者和癫痫患者体内该酶水平升高。在甲状腺功能亢进患者中，碳酸酐酶B与T3树脂摄取或蛋白结合碘（PBI）水平之间存在显著负相关。同时，采用免疫吸附法对上述患者体内碳酸酐酶B依赖性酯酶活性（活性碳酸酐酶B酶）进行动力学测定。将所得结果与通过单扩散免疫比浊技术估算的总酶蛋白（活性和非活性碳酸酐酶B酶）进行比较。在接受乙酰唑胺治疗的患者中，碳酸酐酶B的“真实”比活性（碳酸酐酶B依赖性酯酶活性/总碳酸酐酶B蛋白）显著降低。在慢性阻塞性肺疾病患者中，未观察到明显变化。另一方面，甲状腺功能亢进患者的比活性升高，比正常受试者高出2倍。文中对这些活性酶的临床意义进行了讨论。
