大鼠肝脏微粒体部分中L-丝氨酸碱基交换酶的磷脂底物特异性
Phospholipid substrate-specificity of the L-serine base-exchange enzyme in rat liver microsomal fraction.
作者信息
Bjerve K S
出版信息
Biochem J. 1984 May 1;219(3):781-4. doi: 10.1042/bj2190781.
Abstract
The specificity of the L-serine base-exchange enzyme towards the fatty acid composition of the phospholipid substrate was investigated with a rat liver microsomal fraction. The relative rates of L-serine incorporation into saturated-hexaenoic, saturated-pentaenoic, saturated-tetraenoic, saturated-trienoic, dienoic-dienoic, monoenoic-dienoic, saturated-dienoic and saturated-monoenoic + saturated-saturated phosphatidylserine molecular species were 42, 5, 23, 4, 5, 4, 5 and 11% respectively. This is similar to, but not identical with, the relative mass abundance of these molecular species in total liver cell phosphatidylserines. The results indicate that the substrate-specificity of the L-serine base-exchange enzyme can at least in part explain the observed fatty acid composition of rat liver phosphatidylserines.
摘要
利用大鼠肝脏微粒体组分研究了L-丝氨酸碱基交换酶对磷脂底物脂肪酸组成的特异性。L-丝氨酸掺入饱和六烯酸、饱和五烯酸、饱和四烯酸、饱和三烯酸、二烯酸-二烯酸、单烯酸-二烯酸、饱和二烯酸以及饱和单烯酸+饱和饱和磷脂酰丝氨酸分子种类的相对速率分别为42%、5%、23%、4%、5%、4%、5%和11%。这与这些分子种类在全肝细胞磷脂酰丝氨酸中的相对质量丰度相似，但并不相同。结果表明，L-丝氨酸碱基交换酶的底物特异性至少可以部分解释所观察到的大鼠肝脏磷脂酰丝氨酸的脂肪酸组成。
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