硫酸软骨素与人类血浆低密度脂蛋白相互作用的氘磁共振研究
Deuterium magnetic resonance study of the interaction between chondroitin sulfate and human plasma low density lipoproteins.
作者信息
Fenske D B, Cushley R J
出版信息
Biochem Biophys Res Commun. 1984 Jun 29;121(3):871-7. doi: 10.1016/0006-291x(84)90758-7.
PMID:6430286
Abstract
[2H]Chondroitin sulfate was prepared by partial N-deacetylation of chondroitin sulfate (via hydrazinolysis) followed by treatment with [2H6]acetic anhydride. 2H NMR spectra of [2H]chondroitin sulfate in the presence of human plasma low density lipoprotein provide evidence for a soluble complex stoichiometry of 3 (and possibly 2) lipoproteins per polysaccharide molecule, and allow a rough estimation of the dissociation constant Kd.
摘要
[2H]硫酸软骨素是通过硫酸软骨素的部分N-脱乙酰化（通过肼解），然后用[2H6]乙酸酐处理而制备的。在人血浆低密度脂蛋白存在下，[2H]硫酸软骨素的2H NMR光谱为每个多糖分子有3个（可能还有2个）脂蛋白的可溶性复合物化学计量提供了证据，并允许对解离常数Kd进行粗略估计。
Zotero 插件