Walton M J, Cowey C B, Adron J W
Br J Nutr. 1984 Jul;52(1):115-22. doi: 10.1079/bjn19840077.
Groups of rainbow trout (Salmo gairdneri; mean weight 5 g) were given diets containing 10, 12, 14, 17, 21, 24 and 26 g lysine/kg diet for 12 weeks. By analysis of the growth values the dietary requirement of lysine in this experiment was found to be 19 g/kg diet. A similar requirement value was obtained from a dose-response curve of expired 14CO2 (following an intraperitoneal injection of L-[U-14C]lysine) v. dietary lysine concentration. Liver concentrations of total lipid and carnitine and activities of lysine-alpha-ketoglutarate reductase (saccharopine dehydrogenase (NADP+, lysine-forming), EC 1.5.1.8) in the liver were not significantly different in fish from the different dietary treatments. Hepatosomatic index, however, was higher in those fish given low levels of dietary lysine.
将虹鳟鱼（鲑鱼；平均体重5克）分组，分别投喂含10、12、14、17、21、24和26克赖氨酸/千克饲料的日粮，持续12周。通过对生长值的分析，发现本实验中赖氨酸的日粮需求量为19克/千克饲料。从呼出的14CO2（腹腔注射L-[U-14C]赖氨酸后）与日粮赖氨酸浓度的剂量反应曲线中获得了类似的需求值。不同日粮处理组鱼肝脏中的总脂质和肉碱浓度以及肝脏中赖氨酸-α-酮戊二酸还原酶（糖碱脱氢酶（NADP+，赖氨酸形成），EC 1.5.1.8）的活性没有显著差异。然而，日粮赖氨酸水平低的鱼的肝体指数较高。
