Lawson D M, Sensui N, Haisenleder D H, Kaufman P N, Gala R R
Breast Cancer Res Treat. 1984;4(2):129-36. doi: 10.1007/BF01806395.
We compared the following parameters in Long-Evans (LE) and Sprague-Dawley (SD) female rats: 1) mammary tumor incidence after DMBA, 2) plasma prolactin (PRL) during the estrous cycle before and after DMBA, 3) plasma PRL in immature females from 0900 hr on day 29 to 0900 hr on day 30, 4) plasma PRL from 1200 to 1700 hr and before and 10 min after i.p. TRH administration in ovariectomized (OVX) rats treated with 200 micrograms polyestradiol phosphate (PEP), 5) anterior pituitary (AP) PRL concentration in OVX rats treated with 200 micrograms PEP, and 6) levels and Sephadex G-100 gel filtration patterns of plasma PRL 10 min after i.p. TRH administration in OVX rats treated with 200 micrograms PEP. We observed marked mammary tumor incidence in SD rats from one supplier (S-SD, Spartan) (96%) compared to SD from another supplier (CR-SD, Charles River) (40%) or LE rats (10%). Plasma PRL was significantly decreased on metestrus-diestrus and increased on proestrus-estrus in SD (both suppliers) but not in LE rats 90 days after DMBA compared to rats not given DMBA and sacrificed at same stages of the estrous cycle on day 55 of age. Immature LE and SD-CR females exhibited significant late afternoon and early morning prolactin surges on days 29-30 whereas SD-H rats had either no surges or poorly synchronized surges at the same times.(ABSTRACT TRUNCATED AT 250 WORDS)
我们比较了长 Evans（LE）和斯普拉格-道利（SD）雌性大鼠的以下参数：1）二甲基苯蒽（DMBA）处理后的乳腺肿瘤发生率；2）DMBA 处理前后发情周期中的血浆催乳素（PRL）水平；3）29 日 09:00 至 30 日 09:00 未成熟雌性大鼠的血浆 PRL 水平；4）用 200 微克磷酸聚雌二醇（PEP）处理的去卵巢（OVX）大鼠腹腔注射促甲状腺激素释放激素（TRH）前 12:00 至 17:00 以及注射后 10 分钟时的血浆 PRL 水平；5）用 200 微克 PEP 处理的 OVX 大鼠垂体前叶（AP）PRL 浓度；6）用 200 微克 PEP 处理的 OVX 大鼠腹腔注射 TRH 后 10 分钟时血浆 PRL 的水平及 Sephadex G - 100 凝胶过滤图谱。我们观察到，来自一个供应商（S - SD，Spartan）的 SD 大鼠乳腺肿瘤发生率显著高于另一个供应商（CR - SD，Charles River）的 SD 大鼠（40%）或 LE 大鼠（10%）（96%）。与未给予 DMBA 且在 55 日龄发情周期相同阶段处死的大鼠相比，DMBA 处理 90 天后，SD 大鼠（两个供应商）在间情期 - 动情后期血浆 PRL 显著降低，在发情前期 - 发情期升高，而 LE 大鼠则无此变化。未成熟的 LE 和 SD - CR 雌性大鼠在 29 - 30 日下午晚些时候和清晨出现显著的催乳素激增，而 SD - H 大鼠在同一时间要么没有激增，要么激增不同步。（摘要截选至 250 字）
