Dony J M, Smals A G, Rolland R, Fauser B C, Thomas C M
Fertil Steril. 1984 Aug;42(2):274-80. doi: 10.1016/s0015-0282(16)48026-x.
Basal serum gonadotropin levels in 11 oligospermic men were significantly higher than in 9 euspermic control subjects, although most were still in the normal range. Basal serum testosterone (T), 17-hydroxyprogesterone (17-OHP), and estradiol levels and their ratios did not differ significantly. Continuous luteinizing hormone-releasing hormone (LH-RH) infusion (1 microgram/minute for 180 minutes) during integrated blood sampling evoked similar gonadotropin responses in both groups but had a differential effect on T: in the control subjects T increased (P less than 0.01) within 15 minutes to 1.5 times baseline, whereas in the oligospermic men T decreased (P less than 0.01). From 60 minutes on, however, T also significantly rose in the oligospermic men, but the maximum increment was about half lower (P less than 0.01) than in the euspermic men, despite virtually similar rises in 17-OHP. Only in the oligospermic men did the 17-OHP/T ratio increase (P less than 0.02) during LH-RH, which is compatible with the occurrence of a 17,20-lyase block. Serum estradiol did not increase in either group. In conclusion, continuous LH-RH infusion uncovers an intrinsic difference in acute Leydig cell stimulation between euspermic and oligospermic men.
11名少精子症男性的基础血清促性腺激素水平显著高于9名正常精子对照组受试者，尽管大多数仍在正常范围内。基础血清睾酮（T）、17-羟孕酮（17-OHP）和雌二醇水平及其比值无显著差异。在综合采血期间持续输注促黄体生成素释放激素（LH-RH）（1微克/分钟，持续180分钟）在两组中引起了相似的促性腺激素反应，但对T有不同影响：在对照组中，T在15分钟内增加（P<0.01）至基线的1.5倍，而在少精子症男性中，T降低（P<0.01）。然而，从60分钟开始，少精子症男性的T也显著升高，但最大增幅比正常精子男性低约一半（P<0.01），尽管17-OHP的升高几乎相似。仅在少精子症男性中，LH-RH期间17-OHP/T比值增加（P<0.02），这与17,20-裂解酶阻滞的发生一致。两组血清雌二醇均未增加。总之，持续输注LH-RH揭示了正常精子和少精子男性在急性睾丸间质细胞刺激方面的内在差异。
