Katz S E, Katz J M, Miller J A, Wang R, Shapiro R
J Assoc Off Anal Chem. 1984 May-Jun;67(3):569-72.
An assay procedure has been developed for virginiamycin in mash-type feeds which includes removing fat interferences from the feeds by a rapid petroleum ether wash, a citric acid extraction, an acetone extraction, and the use of the pad-plate diffusion system. The procedure eliminates the need for compensatory curves prepared from feeds of the same or similar composition. Recoveries from 10 laboratory-prepared feeds containing 11.0 micrograms virginiamycin/g averaged 89.3% with a coefficient of variation (CV) of 5.76%. Recoveries from a laboratory-prepared feed containing 13.2 micrograms virginiamycin/g averaged 89.9% (CV 9.92%). The procedure is simple, reasonably accurate, and precise.
已开发出一种用于测定糊状饲料中维吉尼亚霉素的分析方法，该方法包括通过快速石油醚洗涤去除饲料中的脂肪干扰物、柠檬酸萃取、丙酮萃取以及使用平板扩散系统。该方法无需从相同或相似组成的饲料制备补偿曲线。从10种实验室制备的含11.0微克维吉尼亚霉素/克的饲料中回收的平均回收率为89.3%，变异系数（CV）为5.76%。从实验室制备的含13.2微克维吉尼亚霉素/克的饲料中回收的平均回收率为89.9%（CV 9.92%）。该方法简单、合理准确且精密。
