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针对1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（一种强效降压和血小板激活的醚连接磷脂）的特异性抗体的制备与表征

Production and characterization of specific antibodies against 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (a potent hypotensive and platelet-activating ether-linked phospholipid).

作者信息

Nishihira J, Ishibashi T, Imai Y

出版信息

J Biochem. 1984 May;95(5):1247-51. doi: 10.1093/oxfordjournals.jbchem.a134728.

DOI:10.1093/oxfordjournals.jbchem.a134728
PMID:6430883
Abstract

Specific antibodies against 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet activating factor, PAF) were raised in rabbits. The anti-PAF IgG significantly agglutinated the PAF antigen and inhibited the platelet aggregation induced by PAF. In the double immunodiffusion test, the anti-PAF IgG specifically produced a single precipitin line with only PAF. Neither the 1-acyl-linked nor the non-acetylated analogue exhibited antigenic properties, which implies that the precise chemical structure is essential for the antigenic expression. Cross-reactivity with various PAF analogues was also examined by the radioimmunoprecipitation technique.

摘要

针对1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（血小板活化因子，PAF）的特异性抗体在兔体内产生。抗PAF IgG显著凝集PAF抗原并抑制PAF诱导的血小板聚集。在双向免疫扩散试验中，抗PAF IgG仅与PAF特异性产生一条沉淀线。1-酰基连接的类似物和未乙酰化的类似物均不表现出抗原特性，这表明精确的化学结构对抗原表达至关重要。还通过放射免疫沉淀技术检测了与各种PAF类似物的交叉反应性。

相似文献

1
Production and characterization of specific antibodies against 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (a potent hypotensive and platelet-activating ether-linked phospholipid).针对1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（一种强效降压和血小板激活的醚连接磷脂）的特异性抗体的制备与表征
J Biochem. 1984 May;95(5):1247-51. doi: 10.1093/oxfordjournals.jbchem.a134728.
2
Antibodies to synthetic platelet-activating factor (1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine) analogues with substituents at the sn-2 position.针对在sn-2位带有取代基的合成血小板激活因子（1-O-烷基-2-O-乙酰基-sn-甘油-3-磷酸胆碱）类似物的抗体。
J Biochem. 1991 Nov;110(5):683-7. doi: 10.1093/oxfordjournals.jbchem.a123641.
3
Specific binding of antibodies to platelet-activating factor (PAF) as demonstrated by thin-layer chromatography/immunostaining.
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Antibody to platelet activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine; PAF).抗血小板活化因子（1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱；PAF）抗体。
J Biochem. 1987 Sep;102(3):451-3. doi: 10.1093/oxfordjournals.jbchem.a122075.
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Production and characterization of antibodies to platelet-activating factor.抗血小板活化因子抗体的制备与特性分析
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引用本文的文献

1
Mass spectrometric evidence for the presence of platelet-activating factor (1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) in human amniotic fluid during labor.分娩过程中人类羊水内存在血小板活化因子（1-0-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）的质谱分析证据。
Lipids. 1984 Dec;19(12):907-10. doi: 10.1007/BF02534724.
2
Phospholipid requirement of alkylglycerol monooxygenase from rat liver microsomes.大鼠肝脏微粒体中烷基甘油单加氧酶对磷脂的需求
Lipids. 1986 Mar;21(3):191-4. doi: 10.1007/BF02534820.
3
Biochemical characterization of acetyl-CoA:1-alkyl-2-lyso-sn-glycero-3-phosphocholine acetyltransferase in rat spleen microsomes.
大鼠脾脏微粒体中乙酰辅酶A：1-烷基-2-溶血-sn-甘油-3-磷酸胆碱乙酰转移酶的生化特性
Lipids. 1987 Mar;22(3):185-9. doi: 10.1007/BF02537300.
4
Synthesis of a PAF immunogen and production of PAF-specific antibodies.血小板活化因子免疫原的合成及血小板活化因子特异性抗体的产生。
Lipids. 1991 Dec;26(12):1130-5. doi: 10.1007/BF02536516.
5
A sensitive and specific radioimmunoassay for platelet-activating factor.
Lipids. 1992 Mar;27(3):206-8. doi: 10.1007/BF02536180.