螺旋藻铁氧化还原蛋白-NADP+还原酶。完整氨基酸序列。

Spirulina ferredoxin-NADP+ reductase. The complete amino acid sequence.

作者信息

Yao Y, Tamura T, Wada K, Matsubara H, Kodo K

出版信息

J Biochem. 1984 May;95(5):1513-6. doi: 10.1093/oxfordjournals.jbchem.a134759.

DOI:10.1093/oxfordjournals.jbchem.a134759

Abstract

The amino acid sequence of ferredoxin-NADP+ oxidoreductase [EC 1.18.1.2, FNR] from Spirulina sp., a blue-green alga, was determined. Spirulina ferredoxin-NADP+ oxidoreductase was composed of 294 amino acid residues and the molecular weight of the holoenzyme was 34,135. An apparent homology of the amino(N)-terminal region was found between ferredoxin-NADP+ reductases from Spirulina and spinach. We also found some sequence similarities in human erythrocyte glutathione reductase and p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens, both of which are NADPH-dependent FAD enzymes.

摘要

测定了蓝藻螺旋藻中ferredoxin-NADP⁺氧化还原酶[EC 1.18.1.2，FNR]的氨基酸序列。螺旋藻ferredoxin-NADP⁺氧化还原酶由294个氨基酸残基组成，全酶分子量为34,135。发现螺旋藻和菠菜的ferredoxin-NADP⁺还原酶在氨基（N）末端区域存在明显的同源性。我们还发现人红细胞谷胱甘肽还原酶和荧光假单胞菌的对羟基苯甲酸羟化酶之间存在一些序列相似性，这两种酶都是依赖NADPH的FAD酶。