铁氧化还原蛋白-NADP+氧化还原酶的巯基：二硫键真的存在吗？

The sulfhydryl groups of ferredoxin-NADP+ oxidoreductases: is a disulfide bond really present?

作者信息

Yao Y, Wada K, Takahashi Y, Katoh S, Matsubara H

出版信息

J Biochem. 1985 Oct;98(4):1079-82. doi: 10.1093/oxfordjournals.jbchem.a135355.

DOI:10.1093/oxfordjournals.jbchem.a135355

Abstract

The sulfhydryl groups of three ferredoxin-NADP+ oxidoreductases [EC 1.18.1.2] (FNR) two from blue-green algae, Spirulina sp. and Synechococcus sp., respectively, and one from spinach, were analyzed by carboxymethylation and titration with sulfhydryl reagents under different conditions. Five sulfhydryl groups in Spirulina and spinach enzymes and four in Synechococcus enzyme were found to be in reduced forms. There was no disulfide bond in any FNR tested. The results in the present experiment contradict those reported by Karplus et al. [Biochemistry 23, 6576-6583 (1984)].

摘要

通过羧甲基化以及在不同条件下用巯基试剂滴定，分析了三种铁氧化还原蛋白-NADP⁺氧化还原酶EC 1.18.1.2的巯基，其中两种分别来自蓝藻螺旋藻属和聚球藻属，一种来自菠菜。结果发现，螺旋藻和菠菜的酶中有五个巯基呈还原形式，聚球藻的酶中有四个巯基呈还原形式。在所测试的任何FNR中均未发现二硫键。本实验结果与卡尔普斯等人[《生物化学》23, 6576 - 6583(1984)]报道的结果相矛盾。